Connective tissues consist of cells and an extracellular matrix. The main function of connective tissues is to provide structural support to organs.
ADIPOSE TISSUE STEREOLOGY PRO
Students: Educators’ Pro Tips for Tough TopicsĪdipose tissue is a specialized type of connective tissue Connective tissue Connective tissues originate from embryonic mesenchyme and are present throughout the body except inside the brain and spinal cord.Fundamentals of Nursing: Clinical Skills.BMI, body mass index CRP, C reactive protein DBP, diastolic blood pressure HDL, high density lipoprotein LDL, low density lipoprotein SBP, systolic blood pressure TG, plasma triglycerides waist, waist circumference. Edge color indicates the correlation sign: red for positive correlations and blue for negative ones. Edge thickness is proportional to the strength of the correlation (CCA) or of the partial correlation (GGM). The red nodes have the highest betweenness and the green nodes the lowest one. Nodes’ colors and font size indicate betweenness centrality. Graphs were laid out using force-based algorithms in Gephi 0.8.2 software. This analysis displays the variables that are connected independently from other variables. Clustering was performed using a spin glass model and simulated annealing.
This original system biology approach provides novel insight in the AT response to weight control by highlighting the central role of myristoleic acid that may account for the beneficial effects of weight loss.Ī sparse Graphical Gaussian Model (GGM) was used to estimate partial correlations in each set of variables and regularized canonical correlation analysis (CCA) was used to assess links between paired sets of variable. This relationship was also observed, after WMD, in the group of women that continued to lose weight. By contrast, after LCD a strong positive relationship between AT myristoleic acid (a fatty acid with low AT level) content and de novo lipogenesis mRNAs was found. AT from women regaining weight displayed growth factors, angiogenesis and proliferation signaling signatures, suggesting unfavorable tissue hyperplasia. Overall, AT showed both constant and phase-specific biological signatures in response to dietary intervention. The resulting networks were analyzed using node clustering, systematic important node extraction and cluster comparisons. It consisted in inferring intra-omic networks with sparse partial correlations and inter-omic networks with regularized canonical correlation analysis and finally combining the obtained omic-specific network in a single global model. A 3 steps approach was used to infer a global model involving the 3 sets of variables. After LCD, individuals were stratified a posteriori according to weight change during WMD. AT fatty acids and mRNA levels were quantified in 135 obese women at baseline, after an 8-week low calorie diet (LCD) and after 6 months of ad libitum weight maintenance diet (WMD). To obtain a systems model, a network approach was used to combine all sets of variables (bio-clinical, fatty acids and mRNA levels) and get an overview of their interactions. To decipher the adipose tissue (AT) response to diet induced weight changes we focused on key molecular (lipids and transcripts) AT species during a longitudinal dietary intervention.
This holistic approach requires systems biology to scrutinize the effects of diet on tissue biology. Nutrigenomics investigates relationships between nutrients and all genome-encoded molecular entities.
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